Multiple-mouse magnetic resonance imaging. by Nicholas Bock

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Magnetic resonance imaging (MRI) has a strong role to play in the characterization of genetically altered mice from transgenic and knockout studies, random mutagenesis programs, and the study of traditional mouse models of disease such as mice bearing tumor xenografts. Since the phenotype of a mouse may be unknown, whole-body three-dimensional imaging at a high resolution is needed to detect abnormalities and quantify their size and shape accurately without partial-volume effects. The imaging time per mouse, however, is on the order of hours to produce adequate signal-to-noise ratios (SNRs) in the images. To overcome the inherently low throughput of MRI, this thesis demonstrates a high-field, live multiple-mouse MRI technique that considerably increases the number of mice imaged without either increasing the imaging time or sacrificing SNR.First, the theory of multiple-mouse imaging is developed and a method for producing the highest throughput in an MRI scanner equipped with multiple radiofrequency coils and receiver channels is evaluated on a low-field clinical MRI scanner. Next, that method is implemented on a high-field animal MRI scanner for imaging the brains of seven live mice at once. Finally, the multiple-mouse MRI scanner is used in a phenotyping study of a transgenic mouse line with abnormalities in brain structure, and in a study of a mouse model of brain cancer with a human glioma xenograft.

Book details

The Physical Object
Pagination117 leaves.
Number of Pages117
ID Numbers
Open LibraryOL21302883M
ISBN 100494077301

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The mouse was the first live animal to be imaged using magnetic resonance imaging (MRI) [1], but it has not been utilized extensively in small animal imaging experiments.

The field of mouse phenotyping with magnetic resonance imaging (MRI) is rapidly growing, motivated by the need for improved tools for characterizing and evaluating mouse models of human disease. MRI is an excellent modality for investigating genetically altered by: Magnetic Resonance Imaging (MRI) Magnetic resonance imaging (MRI) is a radiological tool used in the diagnosis of internal diseases in soft tissue, such as the brain and heart.

In addition to excellent soft tissue contrast, the energy used in MRI is not harmful as found in X-ray.

Abstract. Magnetic resonance imaging (MRI) is an essential tool in neuroscience investigations employing laboratory animals. In this chapter, the basic physics of nuclear magnetic resonance (NMR) signals are briefly described, followed by the principle of. The field of mouse phenotyping with magnetic resonance imaging (MRI) is rapidly growing, with both MRI physicists and biologists starting to use MRI to identify mouse models of human disease.

The purpose of this chapter is to provide details of the animal handling necessary for routine and robust in vivo imaging with particular emphasis on Cited by: 5. Using in vivo multiple-mouse magnetic resonance imaging for increased throughput, we imaged groups of cdf mutant, heterozygous, and wild-type mice and made an atlas-based segmentation of the.

using mouse models (). Magnetic resonance imaging (MRI) can be a powerful tool in the analysis of mouse models by providing an in vivo imaging modality that offers soft tissue contrast and high resolution capability ().

Compared to clinical imaging, high field strengths and longerAuthor: Marc Carias. Overview of Magnetic Resonance Imaging. To appreciate the opportunities and limitations of MRM, a basic introduction to the fundamentals of magnetic resonance imaging is useful (Bushberg ; Haacke ; Hornak ).First, it is important to understand the source of signal in MRI, which originates from the tiny nuclear magnetic moments of the constituent atoms and molecules that make up the Cited by: Summary.

Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary Multiple-mouse magnetic resonance imaging.

book achieve high-throughput phenotyping of genetically altered mice using multiple-mouse by: Magnetic resonance imaging for detection and analysis of mouse phenotypes Brian J. Nieman,1,2* Nicholas A.

Bock,1,2 Jonathon Bishop,1 X. Josette Chen,1,2 John G. Sled,1,2 Janet Rossant3,4 and R. Mark Henkelman1,2 1Mouse Imaging Centre, Hospital for Sick Children, Toronto, Canada 2Department of Medical Biophysics, University of Toronto, Toronto.

Study of the Murine Multiple-mouse magnetic resonance imaging. book Mechanical Function Using Magnetic Resonance Imaging: The Current Status, Challenges, and Future Perspectives, Practical Applications in Biomedical Engineering, Adriano O.

Andrade, Adriano Alves Pereira, Eduardo L. Naves and Alcimar B. Soares, IntechOpen, DOI: / Available from:Cited by: 2. Trisomy 16 is the most common (one third) autosomal trisomy found in abortuses.

Interestingly, trisomy 16 in abortuses shows little association with increasing maternal age, suggesting that an unusual age-independent mechanism is responsible for this extraordinarily common trisomic condition. Abstract. The purpose of this book chapter is to provide a basic introduction to the field of magnetic resonance imaging (MRI) and its extension to small animal imaging using magnetic resonance microscopy (MRM).Cited by: 5.

Michael Zerin, in Pediatric Urology, Intravenous Urography. IVU has been all but replaced in pediatric imaging by other modalities, including US, renal scintigraphy, computed tomography (CT), and magnetic resonance urography, with few indications for the examination remaining.

In fact, because of the widespread availability of CT, few pediatric radiology departments in the United. Multimodal magnetic resonance and near-infrared-fluorescent imaging of intraperitoneal ovarian cancer using a dual-mode-dual-gadolinium liposomal contrast agent.

Sci. The trial did not meet its primary biomarker endpoint as measured using multi-parametric magnetic resonance imaging (LiverMultiScan (R), Perspectum Diagnostics). The trial also did not meet secondary endpoints that measure liver stiffness as a surrogate for fibrosis using, magnetic resonance-elastography and FibroScan ® score.

We, and many. For the in vivo imaging of sebaceous glands in living mice (Fig. 1d and Supplementary Movies 1–3), the two-photon absorption by blood cells is independent of Cited by: The omega-3 polyunsaturated fatty acid docosahexaenoic acid (DHA) is implicated in the regulation of both lipid and carbohydrate metabolism.

Thus, we questioned whether dietary DHA and low or high content of sucrose impact on metabolism in mice deficient for elongation of very long-chain fatty acids 2 (ELOVL2), an enzyme involved in the endogenous DHA : Anna M.

Pauter, Alexander W. Fischer, Tore Bengtsson, Abolfazl Asadi, Emanuela Talamonti, Anders Jac. Ivanova E, Roberts R, Bissig D, Pan Z-H, Berkowitz BA. Retinal channelrhodopsinmediated activity in vivo evaluated with manganese-enhanced magnetic resonance imaging.

Mol Vis ; [PMID: ]Author: Subrata Batabyal, Sivakumar Gajjeraman, Sulagna Bhattacharya, Weldon Wright, Samarendra Mohanty. Chronic stress can impair the health of human brains. An important strategy that may prevent the accumulation of stress may be the consumption of functional foods.

When senescence-accelerated mice prone 10 (SAMP10), a stress-sensitive strain, were loaded with stress using imposed male mouse territoriality, brain volume decreased. However, in mice that ingested theanine (6 mg/kg), the main Author: Keiko Unno, Akira Sumiyoshi, Tomokazu Konishi, Michiko Hayashi, Kyoko Taguchi, Yoshio Muguruma, Koic.

Autism spectrum disorder is a neurodevelopmental disorder characterized by deficits in social cognition at its core. Human and animal studies converge in the existence of a network of key brain structures involved in the perception, integration, and coding of social cues.

These structures mainly involve areas traditionally associated with cognitive function, such as the prefrontal cortex Author: Marta Fernández, Teresa Sierra-Arregui, Olga Peñagarikano. Journal of Magnetic Resonance Imaging. An acute dose of gamma-hydroxbutyric acid (GHB) alters gene expression in multiple mouse brains.

Magnetic Resonance in Food. 7: Magnetic Resonance Spectroscopy (Spielman) Didic et al., Magnetic Resonance Spectroscopic Imaging detects metabolic changes within the medial temporal lobe in aMCI (see:).

Westman et al., Resonance Imaging and Magnetic Resonance Spectroscopy for detection of early Alzheimer's disease. Magnetic Resonance Imaging (MRI) of Mouse Heart Structure and Activity: MRI imaging of mice was performed in the UPMC Children’s Hospital Animal Imaging Core under isoflurane anesthesia according to their standard protocol using a 7T micro magnetic resonance imaging msyste (Bruker micro-MRI, BioSpin 70/ Scanning time for each mouse).

Inspect the premises carefully and look out for signs of damage caused by gnawing or feeding, holes, smears and droppings. Mice tend to feed by nibbling, eating the outside of grains and leaving the core.

However, rats will often leave crumbs or smaller pieces of food. House mice tend to live inside the building and will gnaw holes up to 20 mm. Using a single domain antibody (sdAb), NM, against PD-L1, radiolabeled site-specifically with technetiumm (99mTc) for single photon emission computed tomography (SPECT) imaging, we aimed to assess the safety, radiation dosimetry and imaging characteristics of this radiopharmaceutical and correlate tumor uptake with PD-L1 Cited by: 7.

SRS microscopic imaging of biosynthetic incorporation of deuterium from D 2 O into macromolecules in living cells and animals. a D 2 O-derived deuterium can form O–D, S–D, and N–D bonds through reversible non-enzymatic H/D exchange and be incorporated into C–D bonds of metabolic precursors for the synthesis of macromolecules through irreversible enzymatic : Min W.

Behind the Connectome Commotion. semi-automated pipeline for imaging multiple mouse brains using light microscopy, a technique whose resolution lies between that of electron microscopy, which is impractical for mammalian brains, and the non-invasive yet far coarser magnetic resonance imaging.

Chamuleau RA, Creyghton JH, De Nie I, et al. Is the magnetic resonance imaging proton spin-lattice relaxation time a reliable noninvasive parameter of developing liver fibrosis.

Hepatology Chamultirat W, Jordan SJ, Mason RP. Nitric oxide production during endotoxic shock in carbon tetrachloride-treated rats. Library resources for researchers using mass spectrometry. "Written by the founders of the SPARK program at Stanford University, this book is a practical guide designed for professors, students and clinicians at academic research institutions who are interested in learning more about the drug development process and how to help their discoveries become the novel drugs of the future.

Our 7th Annual Neuroscience Virtual Event is now available On-Demand!The event will remain open 6 months from the date of the live event.

The webinars will be available for unlimited on-demand viewing. The theme of this year’s event was the Biological Basis of brain and nervous system are the physical base underlying all cognitive and motor behaviors. Poster Session II-Tuesday. with enlarged lateral ventricles measured using magnetic resonance imaging methods.

In male D/+ mice behavioral. The subtle hypoxia underlying chronic cardiovascular disease is an attractive target for PET imaging, but the lead hypoxia imaging agents 64CuATSM and 18FMISO are trapped only at extreme levels of hypoxia and hence are insufficiently sensitive for this by:   Using an 11 C-PiB (Pittsburgh Compound B) magnetic resonance imaging (MRI) molecular probe, Villemagne et al.

demonstrated that human subjects with high rates of probe retention (and therefore higher rates of Aβ deposition) had higher rates of memory loss, cortical gray matter loss, and hippocampal atrophy than those subjects with low probe Cited by: VOL.

Doctor-assisted suicide magnetic resonance imaging, digital fluoroscopy, digital mammography, colour Doppler ultrasonography and a picture archiving and communication system in.

magnetic resonance imaging in normal and failing mouse hearts on a vertical T system. J Magn Reson Imaging. 5. Schneider JE, Wiesmann F, Lygate CA, et al. How to perform an accurate assessment of cardiac function in mice using high-resolution magnetic resonance imaging.

J Cardiovasc Magn Reson. 8:: Sarah A Detombe. Abstract. Advancement of microscopic technologies established significant progress in our understanding of the brain. In the recent effort to elucidate the complete wiring map of the brain circuitry termed ‘connectome’, the different modalities of imaging technology, including those of light and electron microscopy, have started providing essential contribution in multiple by: 9.

Collagen is the most abundant structural protein in the human body. When it is excited by femtosecond near infrared laser, second harmonic generation (SHG) signal at half the wavelength of the excitation wave is excited.

For imaging thick tissues, the SHG signal is collected in the backward direction. The objective of this work is to elaborate the origin of the backward SHG in collagen at the. One compound, termedwas found to bind to RAS proteins using microscale thermophoresis, nuclear magnetic resonance spectroscopy, and isothermal titration calorimetry and to exhibit lethality.

Magnetic resonance imaging conditionally safe neurostimulation leads: Investigation of the maximum safe lead tip temperature. Neurosurgery ; 74 (2) Cohen Y, Alchanatis V, Sela E, Saranga Y, Cohen S, Meron M, Bosak A, Tsipris J, Ostrovsky V, Orolov V, Levj A, Brikman R.

Crop water status estimation using thermography: multi-year. To evaluate the reliability of the 18F-FDG PET/CT-based peri-tumoral halo uptake layer (PHL) method for assessing tumor size, a total of 79 female patients with breast cancer who underwent 18F-FDG PET/CT, breast ultrasound and magnetic resonance imaging (MRI) evaluations were included in the present study.Fast eigenvector centrality mapping of voxel-wise connectivity in functional magnetic resonance imaging: implementation, validation, and interpretation.

Brain Connectivity, 2(5), Magnetic resonance imaging: Organisms Homo sapiens: Diseases and multiple mouse tissues By continuing to browse this site, you agree to allow omicX and its partners to use cookies to analyse the site's operation and effectiveness, to display ads tailored to your interests and to provide you with relevant promotional messages and other.

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